<?xml version="1.1" encoding="utf-8"?>
<article xsi:noNamespaceSchemaLocation="http://jats.nlm.nih.gov/publishing/1.1/xsd/JATS-journalpublishing1-mathml3.xsd" dtd-version="1.1" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"><front><journal-meta><journal-id journal-id-type="publisher-id">EAE</journal-id><journal-title-group><journal-title>Environment and Ecology</journal-title></journal-title-group><issn>2998-9094</issn><eissn>2998-9108</eissn><publisher><publisher-name>Art and Technology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.61369/EAE.2026030007</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title>营养生长阶段的大豆镍胁迫响应：生长与生理特征</title><url>https://artdesignp.com/journal/EAE/3/3/10.61369/EAE.2026030007</url><author>冯天畅,周选泽,戚文华</author><pub-date pub-type="publication-year"><year>2026</year></pub-date><volume>3</volume><issue>3</issue><history><date date-type="pub"><published-time>2026-06-20</published-time></date></history><abstract>为探究营养生长阶段镍胁迫对大豆的生理毒性效应，本研究以中豆7901大豆为材料，设置土壤镍浓度0、30、60、120、240 mg/kg，测定分枝期植株生物量、叶片氧化损伤及抗氧化酶活性。结果显示：随镍浓度升高，大豆根茎叶生长受显著抑制，根在30mg/kg时重量略有上升然后随浓度进一步升高而持续下降，茎叶随浓度升高持续下降；叶片H₂O₂ 与MDA含量持续上升，氧化应激与膜脂过氧化加剧；SOD、CAT活性先升后降，30 mg/kg达峰值，高浓度下酶系统受损。研究表明，大豆营养生长期对镍胁迫的耐受临界浓度为30 ～ 60 mg/kg，240 mg/kg超出耐受阈值；SOD‑CAT级联清除是大豆响应镍胁迫的核心抗氧化机制。本研究为耐镍大豆品种选育与农田镍污染风险评估提供理论依据。</abstract><keywords>大豆,镍胁迫,抗氧化酶,生理响应</keywords></article-meta></front><body/><back><ref-list><ref id="B1" content-type="article"><label>1</label><element-citation publication-type="journal"><p>[1] 王鹏新. 土壤和植物中的镍及其相互关系 [J]. 农业环境科学学报, 1993, (5): 213&amp;ndash;6,2.[2] KUMAR A, JIGYASU D K, SUBRAHMANYAM G, et al. Nickel in terrestrial biota: Comprehensive review on contamination, toxicity, tolerance and its remediation approaches [J]. Chemosphere, 2021, 275: 129996.[3] 闫巧俐, 华震宇, 何伟忠, 等. 枣果中重金属镍来源途径探究 [J]. 食品与机械, 2023, (1): 95&amp;ndash;9.[4] 崔德杰, 张玉龙. 土壤重金属污染现状与修复技术研究进展 [J]. 土壤通报, 2004, 35(003): 366&amp;ndash;70.[5] REHMAN M, ALI B, SALAM A, 等. Nickel Stress Modulates Growth Dynamics, Disrupts Redox Homeostasis, and Induces Ultrastructural Damage in Maize [J]. Crop Design, 2025: 100122.[6] 刘国栋. 植物营养元素&amp;mdash;Ni [J]. 植物营养与肥料学报, 2001, 7(1): 103&amp;ndash;8.[7] DE PAULA CORREIA D V, RODAK B W, MACHADO H A, et al. Beneficial or detrimental? How nickel application alters the ionome of soybean plants [J]. Plant Science, 2024, 349: 112274.[8] SADEGHIPOUR O. Chitosan application improves nickel toxicity tolerance in soybean [J]. Journal of Soil Science and Plant Nutrition, 2021, 21(3): 2096&amp;ndash;104.[9] HU Z-Y, DENG X-B, PENG X-X, et al. Effects of external calcium on activities of antioxidant enzymes and membrane lipid peroxidation in rice seedlings under nickel stress [J]. Chinese Journal of Rice Science, 2007, 21(4): 367.[10] JOMOVA K, ALOMAR S Y, ALWASEL S H, et al. Several lines of antioxidant defense against oxidative stress: antioxidant enzymes, nanomaterials with multiple enzyme-mimicking activities, and low-molecular-weight antioxidants [J]. Archives of toxicology, 2024, 98(5): 1323&amp;ndash;67.[11] SHENG Y, ABREU I A, CABELLI D E, et al. Superoxide dismutases and superoxide reductases [J]. Chemical reviews, 2014, 114(7): 3854&amp;ndash;918.[12] NAZIR F, FARIDUDDIN Q, KHAN T A. Hydrogen peroxide as a signalling molecule in plants and its crosstalk with other plant growth regulators under heavy metal stress [J]. Chemosphere, 2020, 252: 126486.[13] AYALA A, MU&amp;ntilde;OZ M F, ARG&amp;uuml;ELLES S. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4 ‐hydroxy ‐2 ‐nonenal [J]. Oxidative medicine and cellular longevity, 2014, 2014(1): 360438.[14] ALSCHER R G, ERTURK N, HEATH L S. Role of superoxide dismutases (SODs) in controlling oxidative stress in plants [J]. Journal of experimental botany, 2002, 53(372): 1331&amp;ndash;41.[15] GILL S S, ANJUM N A, GILL R, et al. Superoxide dismutase&amp;mdash;mentor of abiotic stress tolerance in crop plants [J]. Environmental science and pollution research, 2015, 22(14): 10375&amp;ndash;94.[16] MHAMDI A, QUEVAL G, CHAOUCH S, et al. Catalase function in plants: a focus on Arabidopsis mutants as stress-mimic models [J]. Journal of experimental botany, 2010, 61(15): 4197&amp;ndash;220.[17] 王启明. 镍对大豆种子萌发和膜脂过氧化作用及体内保护酶活性的影响 [J]. 种子, 2006, 25(7): 9&amp;ndash;12.[18] 赵娜. 镍胁迫对玉米、大豆苗期生长及生理特性的影响 [D]. 吉林农业大学, 2011.[19] CUYPERS A, HENDRIX S, AMARAL DOS REIS R, et al. Hydrogen peroxide, signaling in disguise during metal phytotoxicity [J]. Frontiers in Plant Science, 2016, 7: 470.</p><pub-id pub-id-type="doi"/></element-citation></ref></ref-list></back></article>
