<?xml version="1.1" encoding="utf-8"?>
<article xsi:noNamespaceSchemaLocation="http://jats.nlm.nih.gov/publishing/1.1/xsd/JATS-journalpublishing1-mathml3.xsd" dtd-version="1.1" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"><front><journal-meta><journal-id journal-id-type="publisher-id">CDCST</journal-id><journal-title-group><journal-title>China Daily Chemical Science Technology</journal-title></journal-title-group><issn>2997-7096</issn><eissn>2997-710X</eissn><publisher><publisher-name>Art and Technology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.61369/CDCST.7989</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title>表面活性剂组合温和性的体外和在体研究</title><url>https://artdesignp.com/journal/CDCST/1/2/10.61369/CDCST.7989</url><author>常晓维,方圆圆,王雨冰,夏丹丹,吴瑶瑶,王奕涵,陈梅</author><pub-date pub-type="publication-year"><year>2024</year></pub-date><volume>1</volume><issue>2</issue><history><date date-type="pub"><published-time>2024-11-25</published-time></date></history><abstract>本研究通过建立体外人角质细胞模型，对6个单一表面活性剂的细胞活力影响进行评估。之后将两性/非离子表活和甲基椰油酰基牛磺酸钠-SMCT进行两元、三元复配并检测体外IC50值和炎症因子IL-1&amp;alpha;水平。结果表明，在单一表活中，非离子表活尤其是海藻糖酯THL的IC50值最高、细胞毒性最低。在表活复配体系中，基于阴离子/两性/非离子表活复配的三元体系产生的IL-1&amp;alpha;水平低于阴离子/两性表活复配的二元体系，且THL显示出比烷基糖苷APG在降低炎症因子方面更好的效果。最后，我们通过单次封闭式斑贴实验对复配体系进行了经皮水分散失率的测试，以及红斑反应评价。并综合分析了复配体系对皮肤脂质屏障的影响和引起的细胞炎症反应，多个因素作用下最终导致皮肤产生红斑等不良反应。</abstract><keywords>表面活性剂 ,温和低刺激 ,细胞毒性 ,炎症因子 ,皮肤反应</keywords></article-meta></front><body/><back><ref-list><ref id="B1" content-type="article"><label>1</label><element-citation publication-type="journal"><p>[1] D' souza p, Rathi S K. Shampoo and conditioners: what a dermatologist should know? [J]. Indian journal of dermatology, 2015, 60(3): 248-54.&amp;nbsp;[2] 王云， 刘武良， 刘培等， 洗发水导致头痒的原因分析及对 策[J]，广东微量元素科学，2013, 20, 67-70.&amp;nbsp;[3] Mueller J J, Wenk H H. Biosurfactants&amp;ndash;Nature's Solution for Today's Cleaning Challenges [J]. Chimia, 2021, 75(9): 752-756.&amp;nbsp;[4] Karnwal A, Shrivastava S, Al-tawaha A R M S, et al. Microbial biosurfactant as an alternate to chemical surfactants for application in cosmetics industries in personal and skin care products: a critical review [J]. BioMed Research International, 2023, 2023(1): 2375223.&amp;nbsp;[5] Adu S A, Naughton P J, Marchant R, et al. Microbial biosurfactants in cosmetic and personal skincare pharmaceutical formulations [J]. Pharmaceutics, 2020, 12(11): 1099.&amp;nbsp;[6] Froebe C, Simion F, Rhein L, et al. Stratum corneum lipid removal by surfactants: relation to in vivo irritation [J]. Dermatology, 1990, 181(4): 277-83.&amp;nbsp;[7] Mukherjee S, Yang L, Vincent C, et al. A comparison between interactions of triglyceride oil and mineral oil with proteins and their ability to reduce cleanser surfactant-induced irritation [J]. International Journal of Cosmetic Science, 2015, 37(4): 371-8.&amp;nbsp;[8] Purohit P, Chandar P, Vilinska A, et al. Effect of mixed surfactants on stratum corneum: a drying stress and R aman spectroscopy study [J]. International Journal of Cosmetic Science, 2014, 36(4): 379-85.&amp;nbsp;[9] Krzyzaniak J F, Yalkowsky S H. Lysis of human red blood cells 3: Effect of contact time on surfactant-induced hemolysis [J]. PDA Journal of Pharmaceutical Science and Technology, 1998, 52(2): 66-9. [10] Welss T, Basketter D A, Schr&amp;ouml;der K R. In vitro skin irritation: facts and future. State of the art review of mechanisms and models [J]. Toxicology in vitro, 2004, 18(3): 231-43.&amp;nbsp;[11] Mizutani T, Mori R, Hirayama M, et al. Sodium lauryl sulfate stimulates the generation of reactive oxygen species through interactions with cell membranes [J]. Journal of oleo science, 2016, 65(12): 993- 1001.&amp;nbsp;[12] Monteiro S A, Sassaki G L, de Souza L M, et al. Molecular and structural characterization of the biosurfactant produced by Pseudomonas aeruginosa DAUPE 614[J]. Chemistry and physics of lipids, 2007, 147(1): 1-13.&amp;nbsp;[13] S&amp;aacute;nchez M, Aranda F J, Espuny M J, et al. Aggregation behaviour of a dirhamnolipid biosurfactant secreted by Pseudomonas aeruginosa in aqueous media[J]. Journal of colloid and interface science, 2007, 307(1): 246-253.&amp;nbsp;[14] Van Hamme J D, Singh A, Ward O P. Physiological aspects: Part 1 in a series of papers devoted to surfactants in microbiology and biotechnology[J]. Biotechnology advances, 2006, 24(6): 604-620.&amp;nbsp;[15] Lang S, Philp J C. Surface-active lipids in rhodococci[J]. Antonie van leeuwenhoek, 1998, 74: 59-70.&amp;nbsp;[16] Marqu&amp;eacute;s A M, Pinazo A, Farfan M, et al. The physicochemical properties and chemical composition of trehalose lipids produced by Rhodococcus erythropolis 51T7[J]. Chemistry and physics of lipids, 2009, 158(2): 110-117.&amp;nbsp;[17] Ward R K, Hubbard A W, Sulley H, et al. Human keratinocyte cultures in an in vitro approach for the assessment of surfactantinduced irritation[J]. Toxicology in vitro, 1998, 12(2): 163-173.&amp;nbsp;[18] Walters R M, Gandolfi L, Mack M C, et al. In vitro assessment of skin irritation potential of surfactant-based formulations by using a 3-D skin reconstructed tissue model and cytokine response[J]. Alternatives to Laboratory Animals, 2016, 44(6): 523-532.</p><pub-id pub-id-type="doi"/></element-citation></ref></ref-list></back></article>
